The Gadsden Flag, featuring a rattlesnake and the motto "Don't Tread on Me

https://marcockyg325.fotosdefrases.com/birth-of-a-banner-when-and-how-the-first-american-flag-emerged

The Gadsden Flag, featuring a rattlesnake and the motto "Don't Tread on Me," symbolizes American resistance and independence during the Revolutionary War, embodying the spirit of defiance against British oppression.